Particle size distribution using image processing in MATLAB
http://jcp.bmj.com/content/62/6/481/F6.large.jpg
I want to plot the number of fat cells in the liver versus their size. For which I wanted to use the following steps.
Apply adaptive thresholding on the gray level image.
Find distance transform on the thresholded image.
Invert the distance transform and assign a high value to the background.
Extract marker using gray scale reconstruction and assign zero value to marker.
Apply watershed transformation on the marker embedded inverted distance image to obtain the segmented image.
Use gray scale reconstruction to find radius of each droplet and subsequently calculate area of each labelled droplet.
In step 3 how do I assign a high value to the background and can you help me with step 4?http://jcp.bmj.com/content/62/6/481/F6.large.jpg
I want to plot the number of fat cells in the liver versus their size. For which I wanted to use the following steps.
Apply adaptive thresholding on the gray level image.
Find distance transform on the thresholded image.
Invert the distance transform and assign a high value to the background.
Extract marker using gray scale reconstruction and assign zero value to marker.
Apply watershed transformation on the marker embedded inverted distance image to obtain the segmented image.
Use gray scale reconstruction to find radius of each droplet and subsequently calculate area of each labelled droplet.
In step 3 how do I assign a high value to the background and can you help me with step 4? http://jcp.bmj.com/content/62/6/481/F6.large.jpg
I want to plot the number of fat cells in the liver versus their size. For which I wanted to use the following steps.
Apply adaptive thresholding on the gray level image.
Find distance transform on the thresholded image.
Invert the distance transform and assign a high value to the background.
Extract marker using gray scale reconstruction and assign zero value to marker.
Apply watershed transformation on the marker embedded inverted distance image to obtain the segmented image.
Use gray scale reconstruction to find radius of each droplet and subsequently calculate area of each labelled droplet.
In step 3 how do I assign a high value to the background and can you help me with step 4? image processing MATLAB Answers — New Questions