Hi,
I’ve been using MATLAB to analyze 3D confocal images saved as TIFF files. My process is to get the original images from NIS-Elements, then open them in ImageJ to crop just the droplet region, and save that as a TIFF. In MATLAB R2022b, this TIFF input would allow the program to do a full 3D reconstruction of the droplet and calculate the data I need.
But now that I’m using R2024b, the same TIFF files don’t load properly. The 3D reconstruction step stops and doesn’t work, and I get no data even when I test it using the exact same TIFF files and inputs that worked previously.
I’m wondering if MATLAB R2024b handles TIFF files differently than R2022b? Are there specific settings I should use when saving TIFFs from ImageJ to make sure they work with the latest MATLAB? Or would this be entirely a different problem?
Thanks!Hi,
I’ve been using MATLAB to analyze 3D confocal images saved as TIFF files. My process is to get the original images from NIS-Elements, then open them in ImageJ to crop just the droplet region, and save that as a TIFF. In MATLAB R2022b, this TIFF input would allow the program to do a full 3D reconstruction of the droplet and calculate the data I need.
But now that I’m using R2024b, the same TIFF files don’t load properly. The 3D reconstruction step stops and doesn’t work, and I get no data even when I test it using the exact same TIFF files and inputs that worked previously.
I’m wondering if MATLAB R2024b handles TIFF files differently than R2022b? Are there specific settings I should use when saving TIFFs from ImageJ to make sure they work with the latest MATLAB? Or would this be entirely a different problem?
Thanks! Hi,
I’ve been using MATLAB to analyze 3D confocal images saved as TIFF files. My process is to get the original images from NIS-Elements, then open them in ImageJ to crop just the droplet region, and save that as a TIFF. In MATLAB R2022b, this TIFF input would allow the program to do a full 3D reconstruction of the droplet and calculate the data I need.
But now that I’m using R2024b, the same TIFF files don’t load properly. The 3D reconstruction step stops and doesn’t work, and I get no data even when I test it using the exact same TIFF files and inputs that worked previously.
I’m wondering if MATLAB R2024b handles TIFF files differently than R2022b? Are there specific settings I should use when saving TIFFs from ImageJ to make sure they work with the latest MATLAB? Or would this be entirely a different problem?
Thanks! tiff, 3d reconstruction, matlab2024b, image proces MATLAB Answers — New Questions

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