function varargout = FLIM_processing(varargin)
% FLIM_PROCESSING MATLAB code for FLIM_processing.fig
% FLIM_PROCESSING, by itself, creates a new FLIM_PROCESSING or raises the existing
% singleton*.
%
% H = FLIM_PROCESSING returns the handle to a new FLIM_PROCESSING or the handle to
% the existing singleton*.
%
% FLIM_processing(‘CALLBACK’,hObject,eventData,handles,…) calls the local
% function named CALLBACK in FLIM_processing.M with the given input arguments.
%
% FLIM_processing(‘Property’,’Value’,…) creates a new FLIM_processing or raises the
% existing singleton*. Starting from the left, property value pairs are
% applied to the GUI before FLIM_processing_OpeningFcn gets called. An
% unrecognized property name or invalid value makes property application
% stop. All inputs are passed to FLIM_processing_OpeningFcn via varargin.
%
% *See GUI Options on GUIDE’s Tools menu. Choose "GUI allows only one
% instance to run (singleton)".
%
% See also: GUIDE, GUIDATA, GUIHANDLES
% Edit the above text to modify the response to help FLIM_processing
% Last Modified by GUIDE v2.5 13-Apr-2021 19:57:16
% Begin initialization code – DO NOT EDIT
gui_Singleton = 1;
gui_State = struct(‘gui_Name’, mfilename, …
‘gui_Singleton’, gui_Singleton, …
‘gui_OpeningFcn’, @FLIM_processing_OpeningFcn, …
‘gui_OutputFcn’, @FLIM_processing_OutputFcn, …
‘gui_LayoutFcn’, [] , …
‘gui_Callback’, []);
if nargin && ischar(varargin{1})
gui_State.gui_Callback = str2func(varargin{1});
end
if nargout
[varargout{1:nargout}] = gui_mainfcn(gui_State, varargin{:});
else
gui_mainfcn(gui_State, varargin{:});
end
% End initialization code – DO NOT EDIT
% — Executes just before FLIM_processing is made visible.
function FLIM_processing_OpeningFcn(hObject, eventdata, handles, varargin)
% This function has no output args, see OutputFcn.
% hObject handle to figure
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% varargin command line arguments to FLIM_processing (see VARARGIN)
% Choose default command line output for FLIM_processing
handles.output = hObject;
% Update handles structure
guidata(hObject, handles);
% UIWAIT makes FLIM_processing wait for user response (see UIRESUME)
% uiwait(handles.figure1);
% — Outputs from this function are returned to the command line.
function varargout = FLIM_processing_OutputFcn(hObject, eventdata, handles)
% varargout cell array for returning output args (see VARARGOUT);
% hObject handle to figure
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Get default command line output from handles structure
varargout{1} = handles.output;
%% Get the NADH Image Button
% — Executes on button press in pushbutton1.
function read_NADH_intensity_Callback(~, ~, handles)
% hObject handle to pushbutton1 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global nadhTextImage;
global maskShowImage;
global nadhImageName;
global nadhFileName;
global nadhPathName;
% Get the location of the image
[nadhFileName, nadhPathName] = uigetfile(‘*.asc’,’Pick an Image’);
nadhTextImage = importdata(strcat(nadhPathName,nadhFileName));
nadhTextImage(nadhTextImage > 1000) = 0;
nadhTextImage(:,245:256)=0;
maskShowImage = nadhTextImage;
nadhImageName = {nadhFileName,’NADH’};
% display the NADH image in axes1
maxIntensityValue = max(nadhTextImage(:));
minIntensityValue = min(nadhTextImage(:));
set(handles.slider3,’Max’,maxIntensityValue);
set(handles.slider3,’Min’,minIntensityValue);
set(handles.text19,’String’,num2str(minIntensityValue));
set(handles.text20,’String’,num2str(maxIntensityValue));
set(handles.text23,’String’,nadhFileName);
axes(handles.axes1);
imagesc(nadhTextImage)
colormap(gray)
colorbar
axes(handles.axes4);
imagesc(maskShowImage)
colormap(gray)
colorbar
%% Get the Mask Image Button
% — Executes on button press in pushbutton4.
function pushbutton4_Callback(hObject, eventdata, handles)
% hObject handle to pushbutton4 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global nadhTextImage;
global maskImage;
global nadhThreshold;
% get the image mask
maskImage = nadhTextImage;
nadhThreshold = get(handles.edit1,’String’);
nadhThreshold = str2double( nadhThreshold );
maskImage(maskImage < nadhThreshold) = 0;
maskImage(maskImage~=0) = 1;
% display the mask Image axes4
axes(handles.axes4);
imagesc(maskImage)
colormap(gray)
colorbar

%% read FAD intensity image
% — Executes on button press in readFADIntensity.
function read_FAD_intensity_Callback(hObject, eventdata, handles)
% hObject handle to readFADIntensity (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global fadTextImage
% Get the location of the image
global fadImageName;
global fadFileName;
global fadPathName;
[fadFileName, fadPathName] = uigetfile(‘*.asc’,’Pick an Image’);
fadImageName = {fadFileName,’FAD’};
fadTextImage = importdata(strcat(fadPathName,fadFileName));
fadTextImage(fadTextImage > 1000) = 0;
fadTextImage(:,245:256)=0;
% display the NADH image in axes1
set(handles.text24,’String’,fadFileName);
axes(handles.axes2);
imagesc(fadTextImage)
colormap(gray)
colorbar
%% Read NADH T1 Image
% — Executes on button press in pushbutton3.
function pushbutton3_Callback(hObject, eventdata, handles)
% hObject handle to pushbutton3 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global nadhT1Image;
% Get the location of the image
[nadhT1FileName, nadhT1PathName] = uigetfile(‘*.asc’,’Pick an Image’);
nadhT1Image = importdata(strcat(nadhT1PathName,nadhT1FileName));
nadhT1Image(nadhT1Image > 8000) = 0;
nadhT1Image(:,245:256)=0;
% display the NADH image in axes7 and show the image name
axes(handles.axes7);
imagesc(nadhT1Image)
colormap(gray)
colorbar
set(handles.text7 , ‘String’, nadhT1FileName);
function edit1_Callback(hObject, eventdata, handles)
% hObject handle to edit1 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit1 as text
% str2double(get(hObject,’String’)) returns contents of edit1 as a double
% — Executes during object creation, after setting all properties.
function edit1_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit1 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
% — Executes during object deletion, before destroying properties.
function edit1_DeleteFcn(hObject, eventdata, handles)
% hObject handle to edit1 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
%% Calculate Redox Ratio Button
% — Executes on button press in pushbutton5.
function pushbutton5_Callback(hObject, eventdata, handles)
% hObject handle to pushbutton5 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global nadhTextImage;
global fadTextImage;
global redoxRatioImage;
redoxRatioImage = nadhTextImage./(nadhTextImage + fadTextImage);
axes(handles.axes6);
imagesc(redoxRatioImage)
colormap(gray)
colorbar
function edit2_Callback(hObject, eventdata, handles)
% hObject handle to edit2 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit2 as text
% str2double(get(hObject,’String’)) returns contents of edit2 as a double
% — Executes during object creation, after setting all properties.
function edit2_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit2 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
% — Executes on button press in pushbutton6.
function pushbutton6_Callback(~, ~, handles)
% hObject handle to pushbutton6 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global nadhTextImage;
global fadTextImage;
global redoxRatioImage;
global maskImage;
global redoxRatio;
nadhIntensityImage = nadhTextImage.* maskImage;
fadIntensityImage = fadTextImage.* maskImage;
axes(handles.axes1);
imagesc(nadhIntensityImage)
colormap(gray)
colorbar
axes(handles.axes2);
imagesc(fadIntensityImage)
colormap(gray)
colorbar
redoxRatioIntensityImage = redoxRatioImage.* maskImage;
axes(handles.axes6);
imagesc(redoxRatioIntensityImage)
colormap(gray)
colorbar
redoxRatio = mean(redoxRatioIntensityImage(redoxRatioIntensityImage~=0),’omitnan’);
set(handles.edit2 , ‘String’, num2str(redoxRatio));
%% Read NADH T2 Image and display
% — Executes on button press in pushbutton7.
function pushbutton7_Callback(hObject, eventdata, handles)
% hObject handle to pushbutton7 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global nadhT2Image;
% Get the location of the image
[nadhT2FileName, nadhT2PathName] = uigetfile(‘*.asc’,’Pick an Image’);
nadhT2Image = importdata(strcat(nadhT2PathName,nadhT2FileName));
nadhT2Image(nadhT2Image > 8000) = 0;
nadhT2Image(:,245:256)=0;
% display the NADH image in axes7 and show the image name
axes(handles.axes8);
imagesc(nadhT2Image)
colormap(gray)
colorbar
set(handles.text8 , ‘String’, nadhT2FileName);
%% Read NADH alpha Image and display
% — Executes on button press in pushbutton8.
function pushbutton8_Callback(hObject, eventdata, handles)
% hObject handle to pushbutton8 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global nadhAlphaImage;
% Get the location of the image
[nadhAlphaFileName, nadhAlphaPathName] = uigetfile(‘*.asc’,’Pick an Image’);
nadhAlphaImage = importdata(strcat(nadhAlphaPathName,nadhAlphaFileName));
nadhAlphaImage(nadhAlphaImage>100) = 0;
nadhAlphaImage(nadhAlphaImage<0) = 0;
nadhAlphaImage(:,245:256) = 0;
% display the NADH image in axes7 and show the image name
axes(handles.axes9);
imagesc(nadhAlphaImage)
colormap(gray)
colorbar
set(handles.text9 , ‘String’, nadhAlphaFileName);
%% Calculate NADH FLIM mean
% — Executes on button press in pushbutton9.
function pushbutton9_Callback(~, eventdata, handles)
% hObject handle to pushbutton9 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global nadhT1Image;
global nadhT2Image;
global nadhAlphaImage;
global maskImage;
global nadhT1Mean;
global nadhT2Mean;
global nadhAlphaMean;
global nadhFLIMMMean;
nadhFLIMMImage = (nadhT1Image.* nadhAlphaImage + nadhT2Image.*(100 – nadhAlphaImage))/100;
nadhFLIMMImageM = nadhFLIMMImage .* maskImage;
nadhAlphaImageM = nadhAlphaImage.* maskImage;
nadhT2ImageM = nadhT2Image .* maskImage;
nadhT1ImageM = nadhT1Image .* maskImage;
nadhAlphaMean = mean(nadhAlphaImageM(nadhAlphaImageM~=0),’omitnan’);
nadhT2Mean = mean(nadhT2ImageM(nadhT2ImageM~=0),’omitnan’);
nadhT1Mean = mean(nadhT1ImageM(nadhT1ImageM~=0),’omitnan’);
nadhFLIMMMean = mean(nadhFLIMMImageM(nadhFLIMMImageM~=0),’omitnan’);
set(handles.edit3 , ‘String’, num2str(nadhT1Mean));
set(handles.edit4 , ‘String’, num2str(nadhT2Mean));
set(handles.edit5 , ‘String’, num2str(nadhAlphaMean));
set(handles.edit6 , ‘String’, num2str(nadhFLIMMMean));
axes(handles.axes7);
imagesc(nadhT1ImageM)
colormap(gray)
colorbar
axes(handles.axes8);
imagesc(nadhT2ImageM)
colormap(gray)
colorbar
axes(handles.axes9);
imagesc(nadhAlphaImageM)
colormap(gray)
colorbar
function edit3_Callback(hObject, eventdata, handles)
% hObject handle to edit3 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit3 as text
% str2double(get(hObject,’String’)) returns contents of edit3 as a double
% — Executes during object creation, after setting all properties.
function edit3_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit3 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
function edit4_Callback(hObject, ~, handles)
% hObject handle to edit4 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit4 as text
% str2double(get(hObject,’String’)) returns contents of edit4 as a double
% — Executes during object creation, after setting all properties.
function edit4_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit4 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
function edit5_Callback(hObject, eventdata, handles)
% hObject handle to edit5 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit5 as text
% str2double(get(hObject,’String’)) returns contents of edit5 as a double
% — Executes during object creation, after setting all properties.
function edit5_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit5 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
function edit6_Callback(hObject, eventdata, handles)
% hObject handle to edit6 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit6 as text
% str2double(get(hObject,’String’)) returns contents of edit6 as a double
% — Executes during object creation, after setting all properties.
function edit6_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit6 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
%% Read FAD T1 image and display
% — Executes on button press in pushbutton10.
function pushbutton10_Callback(hObject, eventdata, handles)
% hObject handle to pushbutton10 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global fadT1Image;
% Get the location of the image
[fadT1FileName, fadT1PathName] = uigetfile(‘*.asc’,’Pick an Image’);
fadT1Image = importdata(strcat(fadT1PathName,fadT1FileName));
fadT1Image(fadT1Image > 8000) = 0;
fadT1Image(fadT1Image < 0) = 0;
fadT1Image(:,245:256)=0;
% display the NADH image in axes7 and show the image name
axes(handles.axes10);
imagesc(fadT1Image)
colormap(gray)
colorbar
set(handles.text10 , ‘String’, fadT1FileName);
% — Executes on button press in pushbutton11.
function pushbutton11_Callback(hObject, ~, handles)
% hObject handle to pushbutton11 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global fadT2Image;
% Get the location of the image
[fadT2FileName, fadT2PathName] = uigetfile(‘*.asc’,’Pick an Image’);
fadT2Image = importdata(strcat(fadT2PathName,fadT2FileName));
fadT2Image(fadT2Image>8000) = 0;
fadT2Image(fadT2Image < 0) = 0;
fadT2Image(:,245:256)=0;
% display the NADH image in axes7 and show the image name
axes(handles.axes11);
imagesc(fadT2Image)
colormap(gray)
colorbar
set(handles.text11 , ‘String’, fadT2FileName);
% — Executes on button press in pushbutton12.
function pushbutton12_Callback(~, ~, handles)
% hObject handle to pushbutton12 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global fadAlphaImage;
% Get the location of the image
[fadAplhaFileName, fadAlphaPathName] = uigetfile(‘*.asc’,’Pick an Image’);
fadAlphaImage = importdata(strcat(fadAlphaPathName,fadAplhaFileName));
fadAlphaImage(fadAlphaImage>100) = 0;
fadAlphaImage(fadAlphaImage<0) = 0;
fadAlphaImage(:,245:256)=0;
% display the NADH image in axes7 and show the image name
axes(handles.axes12);
imagesc(fadAlphaImage)
colormap(gray)
colorbar
set(handles.text12 , ‘String’, fadAplhaFileName);
% — Executes on button press in pushbutton13.
function pushbutton13_Callback(~, eventdata, handles)
% hObject handle to pushbutton13 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global fadT1Image;
global fadT2Image;
global fadAlphaImage;
global maskImage
global fadT1Mean;
global fadT2Mean;
global fadAlphaMean;
global fadFLIMMMean;
fadFLIMMImage = (fadT1Image.* fadAlphaImage + fadT2Image.*(100 – fadAlphaImage))/100;
fadFLIMMImageM = fadFLIMMImage .* maskImage;
fadAlphaImageM = fadAlphaImage.* maskImage;
fadT2ImageM = fadT2Image .* maskImage;
fadT1ImageM = fadT1Image .* maskImage;
fadAlphaMean = mean(fadAlphaImageM(fadAlphaImageM~=0),’omitnan’);
fadT2Mean = mean(fadT2ImageM(fadT2ImageM~=0),’omitnan’);
fadT1Mean = mean(fadT1ImageM(fadT1ImageM~=0),’omitnan’);
fadFLIMMMean = mean(fadFLIMMImageM(fadFLIMMImageM~=0),’omitnan’);
set(handles.edit7 , ‘String’, num2str(fadT1Mean));
set(handles.edit8 , ‘String’, num2str(fadT2Mean));
set(handles.edit9 , ‘String’, num2str(fadAlphaMean));
set(handles.edit10 , ‘String’, num2str(fadFLIMMMean));
axes(handles.axes10);
imagesc(fadT1ImageM)
colormap(gray)
colorbar
axes(handles.axes11);
imagesc(fadT2ImageM)
colormap(gray)
colorbar
axes(handles.axes12);
imagesc(fadAlphaImageM)
colormap(gray)
colorbar
function edit7_Callback(hObject, eventdata, handles)
% hObject handle to edit7 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit7 as text
% str2double(get(hObject,’String’)) returns contents of edit7 as a double
% — Executes during object creation, after setting all properties.
function edit7_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit7 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
function edit8_Callback(hObject, eventdata, handles)
% hObject handle to edit8 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit8 as text
% str2double(get(hObject,’String’)) returns contents of edit8 as a double
% — Executes during object creation, after setting all properties.
function edit8_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit8 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
function edit9_Callback(hObject, eventdata, handles)
% hObject handle to edit9 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit9 as text
% str2double(get(hObject,’String’)) returns contents of edit9 as a double
% — Executes during object creation, after setting all properties.
function edit9_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit9 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
function edit10_Callback(hObject, eventdata, handles)
% hObject handle to edit10 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit10 as text
% str2double(get(hObject,’String’)) returns contents of edit10 as a double
% — Executes during object creation, after setting all properties.
function edit10_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit10 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
function edit11_Callback(~, eventdata, handles)
% hObject handle to edit11 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit11 as text
% str2double(get(hObject,’String’)) returns contents of edit11 as a double
% — Executes during object creation, after setting all properties.
function edit11_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit11 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
function edit12_Callback(hObject, eventdata, handles)
% hObject handle to edit12 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit12 as text
% str2double(get(hObject,’String’)) returns contents of edit12 as a double
% — Executes during object creation, after setting all properties.
function edit12_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit12 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
function edit13_Callback(hObject, eventdata, handles)
% hObject handle to edit13 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit13 as text
% str2double(get(hObject,’String’)) returns contents of edit13 as a double
% — Executes during object creation, after setting all properties.
function edit13_CreateFcn(hObject, ~, handles)
% hObject handle to edit13 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
%% Select the file to save
% — Executes on button press in pushbutton15.
function pushbutton15_Callback(hObject, eventdata, handles)
% hObject handle to pushbutton15 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global saveExcelLocation;
[saveExcelName, saveExcelPath] = uigetfile(‘*.xlsx’,’Pick an excel file’);
saveExcelLocation = strcat(saveExcelPath,saveExcelName);
set(handles.edit14 , ‘String’, saveExcelLocation);
function edit14_Callback(hObject, eventdata, handles)
% hObject handle to edit14 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit14 as text
% str2double(get(hObject,’String’)) returns contents of edit14 as a double
% — Executes during object creation, after setting all properties.
function edit14_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit14 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
%% Save the data to the excel file
% — Executes on button press in pushbutton16.
function pushbutton16_Callback(hObject, eventdata, handles)
% hObject handle to pushbutton16 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global nadhT1Mean;
global nadhT2Mean;
global nadhAlphaMean;
global nadhFLIMMMean;
global fadT1Mean;
global fadT2Mean;
global fadAlphaMean;
global fadFLIMMMean;
global redoxRatio;
global nadhThreshold;
global saveExcelLocation;
global nadhImageName ;
global fadImageName;
nadhFlimData = [nadhThreshold,redoxRatio,nadhT1Mean,nadhT2Mean,nadhAlphaMean,nadhFLIMMMean];
fadFlimData = [fadT1Mean,fadT2Mean,fadAlphaMean,fadFLIMMMean];
excelData = xlsread(saveExcelLocation,’Sheet1′,’E:E’); % read the column of interest
lastRowNumber = length(excelData)+ 2;
nadhWriteLoc = strcat(‘C’,num2str(lastRowNumber));
nadhNameWriteLoc = strcat(‘A’,num2str(lastRowNumber));
% save NADH data to the excel file
xlswrite(saveExcelLocation,nadhFlimData,’sheet1′,nadhWriteLoc);
xlswrite(saveExcelLocation,nadhImageName,’sheet1′,nadhNameWriteLoc);
% save FAD data to the excel file
lastRowNumber = lastRowNumber + 1;
fadWriteLoc = strcat(‘E’,num2str(lastRowNumber));
fadNameWriteLoc = strcat(‘A’,num2str(lastRowNumber));
xlswrite(saveExcelLocation,fadFlimData,’sheet1′,fadWriteLoc);
xlswrite(saveExcelLocation,fadImageName,’sheet1′,fadNameWriteLoc);
% — Executes on slider movement.
function slider3_Callback(hObject, eventdata, handles)
% hObject handle to slider3 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’Value’) returns position of slider
% get(hObject,’Min’) and get(hObject,’Max’) to determine range of slider
global nadhThreshold;
global maskShowImage;
global nadhTextImage
nadhThreshold = get(handles.slider3 ,’Value’);
% get the image mask
maskShowImage = nadhTextImage;
maskShowImage(maskShowImage < nadhThreshold) = 0;
set(handles.edit1 , ‘String’, num2str(nadhThreshold));
axes(handles.axes4);
imagesc(maskShowImage)
colormap(gray)
colorbar
% — Executes during object creation, after setting all properties.
function slider1_CreateFcn(hObject, eventdata, handles)
% hObject handle to slider3 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: slider controls usually have a light gray background.
if isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,[.9 .9 .9]);
end
function slider3_CreateFcn(hObject, eventdata, handles)
% hObject handle to slider3 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: slider controls usually have a light gray background.
if isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,[.9 .9 .9]);
end
% — Executes on button press in pushbutton17.
function pushbutton17_Callback(hObject, eventdata, handles)
% hObject handle to pushbutton17 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global nadhFileName;
global nadhPathName;
global nadhT1Image;
% Get the location of the image
nadhT1FileName = strrep(nadhFileName,’photons’,’t1′);
nadhT1PathName = nadhPathName;
nadhT1Image = importdata(strcat(nadhT1PathName,nadhT1FileName));
nadhT1Image(nadhT1Image > 8000) = 0;
nadhT1Image(:,245:256)=0;
% display the NADH image in axes7 and show the image name
axes(handles.axes7);
imagesc(nadhT1Image)
colormap(gray)
colorbar
set(handles.text7 , ‘String’, nadhT1FileName);
global nadhT2Image;
% Get the location of the image
nadhT2FileName = strrep(nadhFileName,’photons’,’t2′);
nadhT2PathName = nadhPathName;
nadhT2Image = importdata(strcat(nadhT2PathName,nadhT2FileName));
nadhT2Image(nadhT2Image > 8000) = 0;
nadhT2Image(:,245:256)=0;
% display the NADH image in axes7 and show the image name
axes(handles.axes8);
imagesc(nadhT2Image)
colormap(gray)
colorbar
set(handles.text8 , ‘String’, nadhT2FileName);
global nadhAlphaImage;
% Get the location of the image
nadhAlphaFileName = strrep(nadhFileName,’photons’,’a1[%]’);
nadhAlphaPathName = nadhPathName;
nadhAlphaImage = importdata(strcat(nadhAlphaPathName,nadhAlphaFileName));
nadhAlphaImage(nadhAlphaImage>100) = 0;
nadhAlphaImage(nadhAlphaImage<0) = 0;
nadhAlphaImage(:,245:256) = 0;
% display the NADH image in axes7 and show the image name
axes(handles.axes9);
imagesc(nadhAlphaImage)
colormap(gray)
colorbar
set(handles.text9 , ‘String’, nadhAlphaFileName);
global fadFileName;
global fadPathName;
global fadT1Image;
% Get the location of the image
fadT1FileName = strrep(fadFileName,’photons’,’t1′);
fadT1PathName = fadPathName;
fadT1Image = importdata(strcat(fadT1PathName,fadT1FileName));
fadT1Image(fadT1Image > 8000) = 0;
fadT1Image(fadT1Image < 0) = 0;
fadT1Image(:,245:256)=0;
% display the NADH image in axes7 and show the image name
axes(handles.axes10);
imagesc(fadT1Image)
colormap(gray)
colorbar
set(handles.text10 , ‘String’, fadT1FileName);
global fadT2Image;
% Get the location of the image
fadT2FileName = strrep(fadFileName,’photons’,’t2′);
fadT2PathName = fadPathName;
fadT2Image = importdata(strcat(fadT2PathName,fadT2FileName));
fadT2Image(fadT2Image>8000) = 0;
fadT2Image(fadT2Image < 0) = 0;
fadT2Image(:,245:256)=0;
% display the NADH image in axes7 and show the image name
axes(handles.axes11);
imagesc(fadT2Image)
colormap(gray)
colorbar
set(handles.text11 , ‘String’, fadT2FileName)
global fadAlphaImage;
% Get the location of the image
fadAplhaFileName = strrep(fadFileName,’photons’,’a1[%]’);
fadAlphaPathName = fadPathName;
fadAlphaImage = importdata(strcat(fadAlphaPathName,fadAplhaFileName));
fadAlphaImage(fadAlphaImage>100) = 0;
fadAlphaImage(fadAlphaImage<0) = 0;
fadAlphaImage(:,245:256)=0;
% display the NADH image in axes7 and show the image name
axes(handles.axes12);
imagesc(fadAlphaImage)
colormap(gray)
colorbar
set(handles.text12 , ‘String’, fadAplhaFileName);function varargout = FLIM_processing(varargin)
% FLIM_PROCESSING MATLAB code for FLIM_processing.fig
% FLIM_PROCESSING, by itself, creates a new FLIM_PROCESSING or raises the existing
% singleton*.
%
% H = FLIM_PROCESSING returns the handle to a new FLIM_PROCESSING or the handle to
% the existing singleton*.
%
% FLIM_processing(‘CALLBACK’,hObject,eventData,handles,…) calls the local
% function named CALLBACK in FLIM_processing.M with the given input arguments.
%
% FLIM_processing(‘Property’,’Value’,…) creates a new FLIM_processing or raises the
% existing singleton*. Starting from the left, property value pairs are
% applied to the GUI before FLIM_processing_OpeningFcn gets called. An
% unrecognized property name or invalid value makes property application
% stop. All inputs are passed to FLIM_processing_OpeningFcn via varargin.
%
% *See GUI Options on GUIDE’s Tools menu. Choose "GUI allows only one
% instance to run (singleton)".
%
% See also: GUIDE, GUIDATA, GUIHANDLES
% Edit the above text to modify the response to help FLIM_processing
% Last Modified by GUIDE v2.5 13-Apr-2021 19:57:16
% Begin initialization code – DO NOT EDIT
gui_Singleton = 1;
gui_State = struct(‘gui_Name’, mfilename, …
‘gui_Singleton’, gui_Singleton, …
‘gui_OpeningFcn’, @FLIM_processing_OpeningFcn, …
‘gui_OutputFcn’, @FLIM_processing_OutputFcn, …
‘gui_LayoutFcn’, [] , …
‘gui_Callback’, []);
if nargin && ischar(varargin{1})
gui_State.gui_Callback = str2func(varargin{1});
end
if nargout
[varargout{1:nargout}] = gui_mainfcn(gui_State, varargin{:});
else
gui_mainfcn(gui_State, varargin{:});
end
% End initialization code – DO NOT EDIT
% — Executes just before FLIM_processing is made visible.
function FLIM_processing_OpeningFcn(hObject, eventdata, handles, varargin)
% This function has no output args, see OutputFcn.
% hObject handle to figure
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% varargin command line arguments to FLIM_processing (see VARARGIN)
% Choose default command line output for FLIM_processing
handles.output = hObject;
% Update handles structure
guidata(hObject, handles);
% UIWAIT makes FLIM_processing wait for user response (see UIRESUME)
% uiwait(handles.figure1);
% — Outputs from this function are returned to the command line.
function varargout = FLIM_processing_OutputFcn(hObject, eventdata, handles)
% varargout cell array for returning output args (see VARARGOUT);
% hObject handle to figure
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Get default command line output from handles structure
varargout{1} = handles.output;
%% Get the NADH Image Button
% — Executes on button press in pushbutton1.
function read_NADH_intensity_Callback(~, ~, handles)
% hObject handle to pushbutton1 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global nadhTextImage;
global maskShowImage;
global nadhImageName;
global nadhFileName;
global nadhPathName;
% Get the location of the image
[nadhFileName, nadhPathName] = uigetfile(‘*.asc’,’Pick an Image’);
nadhTextImage = importdata(strcat(nadhPathName,nadhFileName));
nadhTextImage(nadhTextImage > 1000) = 0;
nadhTextImage(:,245:256)=0;
maskShowImage = nadhTextImage;
nadhImageName = {nadhFileName,’NADH’};
% display the NADH image in axes1
maxIntensityValue = max(nadhTextImage(:));
minIntensityValue = min(nadhTextImage(:));
set(handles.slider3,’Max’,maxIntensityValue);
set(handles.slider3,’Min’,minIntensityValue);
set(handles.text19,’String’,num2str(minIntensityValue));
set(handles.text20,’String’,num2str(maxIntensityValue));
set(handles.text23,’String’,nadhFileName);
axes(handles.axes1);
imagesc(nadhTextImage)
colormap(gray)
colorbar
axes(handles.axes4);
imagesc(maskShowImage)
colormap(gray)
colorbar
%% Get the Mask Image Button
% — Executes on button press in pushbutton4.
function pushbutton4_Callback(hObject, eventdata, handles)
% hObject handle to pushbutton4 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global nadhTextImage;
global maskImage;
global nadhThreshold;
% get the image mask
maskImage = nadhTextImage;
nadhThreshold = get(handles.edit1,’String’);
nadhThreshold = str2double( nadhThreshold );
maskImage(maskImage < nadhThreshold) = 0;
maskImage(maskImage~=0) = 1;
% display the mask Image axes4
axes(handles.axes4);
imagesc(maskImage)
colormap(gray)
colorbar

%% read FAD intensity image
% — Executes on button press in readFADIntensity.
function read_FAD_intensity_Callback(hObject, eventdata, handles)
% hObject handle to readFADIntensity (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global fadTextImage
% Get the location of the image
global fadImageName;
global fadFileName;
global fadPathName;
[fadFileName, fadPathName] = uigetfile(‘*.asc’,’Pick an Image’);
fadImageName = {fadFileName,’FAD’};
fadTextImage = importdata(strcat(fadPathName,fadFileName));
fadTextImage(fadTextImage > 1000) = 0;
fadTextImage(:,245:256)=0;
% display the NADH image in axes1
set(handles.text24,’String’,fadFileName);
axes(handles.axes2);
imagesc(fadTextImage)
colormap(gray)
colorbar
%% Read NADH T1 Image
% — Executes on button press in pushbutton3.
function pushbutton3_Callback(hObject, eventdata, handles)
% hObject handle to pushbutton3 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global nadhT1Image;
% Get the location of the image
[nadhT1FileName, nadhT1PathName] = uigetfile(‘*.asc’,’Pick an Image’);
nadhT1Image = importdata(strcat(nadhT1PathName,nadhT1FileName));
nadhT1Image(nadhT1Image > 8000) = 0;
nadhT1Image(:,245:256)=0;
% display the NADH image in axes7 and show the image name
axes(handles.axes7);
imagesc(nadhT1Image)
colormap(gray)
colorbar
set(handles.text7 , ‘String’, nadhT1FileName);
function edit1_Callback(hObject, eventdata, handles)
% hObject handle to edit1 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit1 as text
% str2double(get(hObject,’String’)) returns contents of edit1 as a double
% — Executes during object creation, after setting all properties.
function edit1_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit1 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
% — Executes during object deletion, before destroying properties.
function edit1_DeleteFcn(hObject, eventdata, handles)
% hObject handle to edit1 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
%% Calculate Redox Ratio Button
% — Executes on button press in pushbutton5.
function pushbutton5_Callback(hObject, eventdata, handles)
% hObject handle to pushbutton5 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global nadhTextImage;
global fadTextImage;
global redoxRatioImage;
redoxRatioImage = nadhTextImage./(nadhTextImage + fadTextImage);
axes(handles.axes6);
imagesc(redoxRatioImage)
colormap(gray)
colorbar
function edit2_Callback(hObject, eventdata, handles)
% hObject handle to edit2 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit2 as text
% str2double(get(hObject,’String’)) returns contents of edit2 as a double
% — Executes during object creation, after setting all properties.
function edit2_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit2 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
% — Executes on button press in pushbutton6.
function pushbutton6_Callback(~, ~, handles)
% hObject handle to pushbutton6 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global nadhTextImage;
global fadTextImage;
global redoxRatioImage;
global maskImage;
global redoxRatio;
nadhIntensityImage = nadhTextImage.* maskImage;
fadIntensityImage = fadTextImage.* maskImage;
axes(handles.axes1);
imagesc(nadhIntensityImage)
colormap(gray)
colorbar
axes(handles.axes2);
imagesc(fadIntensityImage)
colormap(gray)
colorbar
redoxRatioIntensityImage = redoxRatioImage.* maskImage;
axes(handles.axes6);
imagesc(redoxRatioIntensityImage)
colormap(gray)
colorbar
redoxRatio = mean(redoxRatioIntensityImage(redoxRatioIntensityImage~=0),’omitnan’);
set(handles.edit2 , ‘String’, num2str(redoxRatio));
%% Read NADH T2 Image and display
% — Executes on button press in pushbutton7.
function pushbutton7_Callback(hObject, eventdata, handles)
% hObject handle to pushbutton7 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global nadhT2Image;
% Get the location of the image
[nadhT2FileName, nadhT2PathName] = uigetfile(‘*.asc’,’Pick an Image’);
nadhT2Image = importdata(strcat(nadhT2PathName,nadhT2FileName));
nadhT2Image(nadhT2Image > 8000) = 0;
nadhT2Image(:,245:256)=0;
% display the NADH image in axes7 and show the image name
axes(handles.axes8);
imagesc(nadhT2Image)
colormap(gray)
colorbar
set(handles.text8 , ‘String’, nadhT2FileName);
%% Read NADH alpha Image and display
% — Executes on button press in pushbutton8.
function pushbutton8_Callback(hObject, eventdata, handles)
% hObject handle to pushbutton8 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global nadhAlphaImage;
% Get the location of the image
[nadhAlphaFileName, nadhAlphaPathName] = uigetfile(‘*.asc’,’Pick an Image’);
nadhAlphaImage = importdata(strcat(nadhAlphaPathName,nadhAlphaFileName));
nadhAlphaImage(nadhAlphaImage>100) = 0;
nadhAlphaImage(nadhAlphaImage<0) = 0;
nadhAlphaImage(:,245:256) = 0;
% display the NADH image in axes7 and show the image name
axes(handles.axes9);
imagesc(nadhAlphaImage)
colormap(gray)
colorbar
set(handles.text9 , ‘String’, nadhAlphaFileName);
%% Calculate NADH FLIM mean
% — Executes on button press in pushbutton9.
function pushbutton9_Callback(~, eventdata, handles)
% hObject handle to pushbutton9 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global nadhT1Image;
global nadhT2Image;
global nadhAlphaImage;
global maskImage;
global nadhT1Mean;
global nadhT2Mean;
global nadhAlphaMean;
global nadhFLIMMMean;
nadhFLIMMImage = (nadhT1Image.* nadhAlphaImage + nadhT2Image.*(100 – nadhAlphaImage))/100;
nadhFLIMMImageM = nadhFLIMMImage .* maskImage;
nadhAlphaImageM = nadhAlphaImage.* maskImage;
nadhT2ImageM = nadhT2Image .* maskImage;
nadhT1ImageM = nadhT1Image .* maskImage;
nadhAlphaMean = mean(nadhAlphaImageM(nadhAlphaImageM~=0),’omitnan’);
nadhT2Mean = mean(nadhT2ImageM(nadhT2ImageM~=0),’omitnan’);
nadhT1Mean = mean(nadhT1ImageM(nadhT1ImageM~=0),’omitnan’);
nadhFLIMMMean = mean(nadhFLIMMImageM(nadhFLIMMImageM~=0),’omitnan’);
set(handles.edit3 , ‘String’, num2str(nadhT1Mean));
set(handles.edit4 , ‘String’, num2str(nadhT2Mean));
set(handles.edit5 , ‘String’, num2str(nadhAlphaMean));
set(handles.edit6 , ‘String’, num2str(nadhFLIMMMean));
axes(handles.axes7);
imagesc(nadhT1ImageM)
colormap(gray)
colorbar
axes(handles.axes8);
imagesc(nadhT2ImageM)
colormap(gray)
colorbar
axes(handles.axes9);
imagesc(nadhAlphaImageM)
colormap(gray)
colorbar
function edit3_Callback(hObject, eventdata, handles)
% hObject handle to edit3 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit3 as text
% str2double(get(hObject,’String’)) returns contents of edit3 as a double
% — Executes during object creation, after setting all properties.
function edit3_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit3 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
function edit4_Callback(hObject, ~, handles)
% hObject handle to edit4 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit4 as text
% str2double(get(hObject,’String’)) returns contents of edit4 as a double
% — Executes during object creation, after setting all properties.
function edit4_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit4 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
function edit5_Callback(hObject, eventdata, handles)
% hObject handle to edit5 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit5 as text
% str2double(get(hObject,’String’)) returns contents of edit5 as a double
% — Executes during object creation, after setting all properties.
function edit5_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit5 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
function edit6_Callback(hObject, eventdata, handles)
% hObject handle to edit6 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit6 as text
% str2double(get(hObject,’String’)) returns contents of edit6 as a double
% — Executes during object creation, after setting all properties.
function edit6_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit6 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
%% Read FAD T1 image and display
% — Executes on button press in pushbutton10.
function pushbutton10_Callback(hObject, eventdata, handles)
% hObject handle to pushbutton10 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global fadT1Image;
% Get the location of the image
[fadT1FileName, fadT1PathName] = uigetfile(‘*.asc’,’Pick an Image’);
fadT1Image = importdata(strcat(fadT1PathName,fadT1FileName));
fadT1Image(fadT1Image > 8000) = 0;
fadT1Image(fadT1Image < 0) = 0;
fadT1Image(:,245:256)=0;
% display the NADH image in axes7 and show the image name
axes(handles.axes10);
imagesc(fadT1Image)
colormap(gray)
colorbar
set(handles.text10 , ‘String’, fadT1FileName);
% — Executes on button press in pushbutton11.
function pushbutton11_Callback(hObject, ~, handles)
% hObject handle to pushbutton11 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global fadT2Image;
% Get the location of the image
[fadT2FileName, fadT2PathName] = uigetfile(‘*.asc’,’Pick an Image’);
fadT2Image = importdata(strcat(fadT2PathName,fadT2FileName));
fadT2Image(fadT2Image>8000) = 0;
fadT2Image(fadT2Image < 0) = 0;
fadT2Image(:,245:256)=0;
% display the NADH image in axes7 and show the image name
axes(handles.axes11);
imagesc(fadT2Image)
colormap(gray)
colorbar
set(handles.text11 , ‘String’, fadT2FileName);
% — Executes on button press in pushbutton12.
function pushbutton12_Callback(~, ~, handles)
% hObject handle to pushbutton12 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global fadAlphaImage;
% Get the location of the image
[fadAplhaFileName, fadAlphaPathName] = uigetfile(‘*.asc’,’Pick an Image’);
fadAlphaImage = importdata(strcat(fadAlphaPathName,fadAplhaFileName));
fadAlphaImage(fadAlphaImage>100) = 0;
fadAlphaImage(fadAlphaImage<0) = 0;
fadAlphaImage(:,245:256)=0;
% display the NADH image in axes7 and show the image name
axes(handles.axes12);
imagesc(fadAlphaImage)
colormap(gray)
colorbar
set(handles.text12 , ‘String’, fadAplhaFileName);
% — Executes on button press in pushbutton13.
function pushbutton13_Callback(~, eventdata, handles)
% hObject handle to pushbutton13 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global fadT1Image;
global fadT2Image;
global fadAlphaImage;
global maskImage
global fadT1Mean;
global fadT2Mean;
global fadAlphaMean;
global fadFLIMMMean;
fadFLIMMImage = (fadT1Image.* fadAlphaImage + fadT2Image.*(100 – fadAlphaImage))/100;
fadFLIMMImageM = fadFLIMMImage .* maskImage;
fadAlphaImageM = fadAlphaImage.* maskImage;
fadT2ImageM = fadT2Image .* maskImage;
fadT1ImageM = fadT1Image .* maskImage;
fadAlphaMean = mean(fadAlphaImageM(fadAlphaImageM~=0),’omitnan’);
fadT2Mean = mean(fadT2ImageM(fadT2ImageM~=0),’omitnan’);
fadT1Mean = mean(fadT1ImageM(fadT1ImageM~=0),’omitnan’);
fadFLIMMMean = mean(fadFLIMMImageM(fadFLIMMImageM~=0),’omitnan’);
set(handles.edit7 , ‘String’, num2str(fadT1Mean));
set(handles.edit8 , ‘String’, num2str(fadT2Mean));
set(handles.edit9 , ‘String’, num2str(fadAlphaMean));
set(handles.edit10 , ‘String’, num2str(fadFLIMMMean));
axes(handles.axes10);
imagesc(fadT1ImageM)
colormap(gray)
colorbar
axes(handles.axes11);
imagesc(fadT2ImageM)
colormap(gray)
colorbar
axes(handles.axes12);
imagesc(fadAlphaImageM)
colormap(gray)
colorbar
function edit7_Callback(hObject, eventdata, handles)
% hObject handle to edit7 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit7 as text
% str2double(get(hObject,’String’)) returns contents of edit7 as a double
% — Executes during object creation, after setting all properties.
function edit7_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit7 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
function edit8_Callback(hObject, eventdata, handles)
% hObject handle to edit8 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit8 as text
% str2double(get(hObject,’String’)) returns contents of edit8 as a double
% — Executes during object creation, after setting all properties.
function edit8_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit8 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
function edit9_Callback(hObject, eventdata, handles)
% hObject handle to edit9 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit9 as text
% str2double(get(hObject,’String’)) returns contents of edit9 as a double
% — Executes during object creation, after setting all properties.
function edit9_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit9 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
function edit10_Callback(hObject, eventdata, handles)
% hObject handle to edit10 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit10 as text
% str2double(get(hObject,’String’)) returns contents of edit10 as a double
% — Executes during object creation, after setting all properties.
function edit10_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit10 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
function edit11_Callback(~, eventdata, handles)
% hObject handle to edit11 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit11 as text
% str2double(get(hObject,’String’)) returns contents of edit11 as a double
% — Executes during object creation, after setting all properties.
function edit11_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit11 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
function edit12_Callback(hObject, eventdata, handles)
% hObject handle to edit12 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit12 as text
% str2double(get(hObject,’String’)) returns contents of edit12 as a double
% — Executes during object creation, after setting all properties.
function edit12_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit12 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
function edit13_Callback(hObject, eventdata, handles)
% hObject handle to edit13 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit13 as text
% str2double(get(hObject,’String’)) returns contents of edit13 as a double
% — Executes during object creation, after setting all properties.
function edit13_CreateFcn(hObject, ~, handles)
% hObject handle to edit13 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
%% Select the file to save
% — Executes on button press in pushbutton15.
function pushbutton15_Callback(hObject, eventdata, handles)
% hObject handle to pushbutton15 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global saveExcelLocation;
[saveExcelName, saveExcelPath] = uigetfile(‘*.xlsx’,’Pick an excel file’);
saveExcelLocation = strcat(saveExcelPath,saveExcelName);
set(handles.edit14 , ‘String’, saveExcelLocation);
function edit14_Callback(hObject, eventdata, handles)
% hObject handle to edit14 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit14 as text
% str2double(get(hObject,’String’)) returns contents of edit14 as a double
% — Executes during object creation, after setting all properties.
function edit14_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit14 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
%% Save the data to the excel file
% — Executes on button press in pushbutton16.
function pushbutton16_Callback(hObject, eventdata, handles)
% hObject handle to pushbutton16 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global nadhT1Mean;
global nadhT2Mean;
global nadhAlphaMean;
global nadhFLIMMMean;
global fadT1Mean;
global fadT2Mean;
global fadAlphaMean;
global fadFLIMMMean;
global redoxRatio;
global nadhThreshold;
global saveExcelLocation;
global nadhImageName ;
global fadImageName;
nadhFlimData = [nadhThreshold,redoxRatio,nadhT1Mean,nadhT2Mean,nadhAlphaMean,nadhFLIMMMean];
fadFlimData = [fadT1Mean,fadT2Mean,fadAlphaMean,fadFLIMMMean];
excelData = xlsread(saveExcelLocation,’Sheet1′,’E:E’); % read the column of interest
lastRowNumber = length(excelData)+ 2;
nadhWriteLoc = strcat(‘C’,num2str(lastRowNumber));
nadhNameWriteLoc = strcat(‘A’,num2str(lastRowNumber));
% save NADH data to the excel file
xlswrite(saveExcelLocation,nadhFlimData,’sheet1′,nadhWriteLoc);
xlswrite(saveExcelLocation,nadhImageName,’sheet1′,nadhNameWriteLoc);
% save FAD data to the excel file
lastRowNumber = lastRowNumber + 1;
fadWriteLoc = strcat(‘E’,num2str(lastRowNumber));
fadNameWriteLoc = strcat(‘A’,num2str(lastRowNumber));
xlswrite(saveExcelLocation,fadFlimData,’sheet1′,fadWriteLoc);
xlswrite(saveExcelLocation,fadImageName,’sheet1′,fadNameWriteLoc);
% — Executes on slider movement.
function slider3_Callback(hObject, eventdata, handles)
% hObject handle to slider3 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’Value’) returns position of slider
% get(hObject,’Min’) and get(hObject,’Max’) to determine range of slider
global nadhThreshold;
global maskShowImage;
global nadhTextImage
nadhThreshold = get(handles.slider3 ,’Value’);
% get the image mask
maskShowImage = nadhTextImage;
maskShowImage(maskShowImage < nadhThreshold) = 0;
set(handles.edit1 , ‘String’, num2str(nadhThreshold));
axes(handles.axes4);
imagesc(maskShowImage)
colormap(gray)
colorbar
% — Executes during object creation, after setting all properties.
function slider1_CreateFcn(hObject, eventdata, handles)
% hObject handle to slider3 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: slider controls usually have a light gray background.
if isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,[.9 .9 .9]);
end
function slider3_CreateFcn(hObject, eventdata, handles)
% hObject handle to slider3 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: slider controls usually have a light gray background.
if isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,[.9 .9 .9]);
end
% — Executes on button press in pushbutton17.
function pushbutton17_Callback(hObject, eventdata, handles)
% hObject handle to pushbutton17 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global nadhFileName;
global nadhPathName;
global nadhT1Image;
% Get the location of the image
nadhT1FileName = strrep(nadhFileName,’photons’,’t1′);
nadhT1PathName = nadhPathName;
nadhT1Image = importdata(strcat(nadhT1PathName,nadhT1FileName));
nadhT1Image(nadhT1Image > 8000) = 0;
nadhT1Image(:,245:256)=0;
% display the NADH image in axes7 and show the image name
axes(handles.axes7);
imagesc(nadhT1Image)
colormap(gray)
colorbar
set(handles.text7 , ‘String’, nadhT1FileName);
global nadhT2Image;
% Get the location of the image
nadhT2FileName = strrep(nadhFileName,’photons’,’t2′);
nadhT2PathName = nadhPathName;
nadhT2Image = importdata(strcat(nadhT2PathName,nadhT2FileName));
nadhT2Image(nadhT2Image > 8000) = 0;
nadhT2Image(:,245:256)=0;
% display the NADH image in axes7 and show the image name
axes(handles.axes8);
imagesc(nadhT2Image)
colormap(gray)
colorbar
set(handles.text8 , ‘String’, nadhT2FileName);
global nadhAlphaImage;
% Get the location of the image
nadhAlphaFileName = strrep(nadhFileName,’photons’,’a1[%]’);
nadhAlphaPathName = nadhPathName;
nadhAlphaImage = importdata(strcat(nadhAlphaPathName,nadhAlphaFileName));
nadhAlphaImage(nadhAlphaImage>100) = 0;
nadhAlphaImage(nadhAlphaImage<0) = 0;
nadhAlphaImage(:,245:256) = 0;
% display the NADH image in axes7 and show the image name
axes(handles.axes9);
imagesc(nadhAlphaImage)
colormap(gray)
colorbar
set(handles.text9 , ‘String’, nadhAlphaFileName);
global fadFileName;
global fadPathName;
global fadT1Image;
% Get the location of the image
fadT1FileName = strrep(fadFileName,’photons’,’t1′);
fadT1PathName = fadPathName;
fadT1Image = importdata(strcat(fadT1PathName,fadT1FileName));
fadT1Image(fadT1Image > 8000) = 0;
fadT1Image(fadT1Image < 0) = 0;
fadT1Image(:,245:256)=0;
% display the NADH image in axes7 and show the image name
axes(handles.axes10);
imagesc(fadT1Image)
colormap(gray)
colorbar
set(handles.text10 , ‘String’, fadT1FileName);
global fadT2Image;
% Get the location of the image
fadT2FileName = strrep(fadFileName,’photons’,’t2′);
fadT2PathName = fadPathName;
fadT2Image = importdata(strcat(fadT2PathName,fadT2FileName));
fadT2Image(fadT2Image>8000) = 0;
fadT2Image(fadT2Image < 0) = 0;
fadT2Image(:,245:256)=0;
% display the NADH image in axes7 and show the image name
axes(handles.axes11);
imagesc(fadT2Image)
colormap(gray)
colorbar
set(handles.text11 , ‘String’, fadT2FileName)
global fadAlphaImage;
% Get the location of the image
fadAplhaFileName = strrep(fadFileName,’photons’,’a1[%]’);
fadAlphaPathName = fadPathName;
fadAlphaImage = importdata(strcat(fadAlphaPathName,fadAplhaFileName));
fadAlphaImage(fadAlphaImage>100) = 0;
fadAlphaImage(fadAlphaImage<0) = 0;
fadAlphaImage(:,245:256)=0;
% display the NADH image in axes7 and show the image name
axes(handles.axes12);
imagesc(fadAlphaImage)
colormap(gray)
colorbar
set(handles.text12 , ‘String’, fadAplhaFileName); function varargout = FLIM_processing(varargin)
% FLIM_PROCESSING MATLAB code for FLIM_processing.fig
% FLIM_PROCESSING, by itself, creates a new FLIM_PROCESSING or raises the existing
% singleton*.
%
% H = FLIM_PROCESSING returns the handle to a new FLIM_PROCESSING or the handle to
% the existing singleton*.
%
% FLIM_processing(‘CALLBACK’,hObject,eventData,handles,…) calls the local
% function named CALLBACK in FLIM_processing.M with the given input arguments.
%
% FLIM_processing(‘Property’,’Value’,…) creates a new FLIM_processing or raises the
% existing singleton*. Starting from the left, property value pairs are
% applied to the GUI before FLIM_processing_OpeningFcn gets called. An
% unrecognized property name or invalid value makes property application
% stop. All inputs are passed to FLIM_processing_OpeningFcn via varargin.
%
% *See GUI Options on GUIDE’s Tools menu. Choose "GUI allows only one
% instance to run (singleton)".
%
% See also: GUIDE, GUIDATA, GUIHANDLES
% Edit the above text to modify the response to help FLIM_processing
% Last Modified by GUIDE v2.5 13-Apr-2021 19:57:16
% Begin initialization code – DO NOT EDIT
gui_Singleton = 1;
gui_State = struct(‘gui_Name’, mfilename, …
‘gui_Singleton’, gui_Singleton, …
‘gui_OpeningFcn’, @FLIM_processing_OpeningFcn, …
‘gui_OutputFcn’, @FLIM_processing_OutputFcn, …
‘gui_LayoutFcn’, [] , …
‘gui_Callback’, []);
if nargin && ischar(varargin{1})
gui_State.gui_Callback = str2func(varargin{1});
end
if nargout
[varargout{1:nargout}] = gui_mainfcn(gui_State, varargin{:});
else
gui_mainfcn(gui_State, varargin{:});
end
% End initialization code – DO NOT EDIT
% — Executes just before FLIM_processing is made visible.
function FLIM_processing_OpeningFcn(hObject, eventdata, handles, varargin)
% This function has no output args, see OutputFcn.
% hObject handle to figure
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% varargin command line arguments to FLIM_processing (see VARARGIN)
% Choose default command line output for FLIM_processing
handles.output = hObject;
% Update handles structure
guidata(hObject, handles);
% UIWAIT makes FLIM_processing wait for user response (see UIRESUME)
% uiwait(handles.figure1);
% — Outputs from this function are returned to the command line.
function varargout = FLIM_processing_OutputFcn(hObject, eventdata, handles)
% varargout cell array for returning output args (see VARARGOUT);
% hObject handle to figure
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Get default command line output from handles structure
varargout{1} = handles.output;
%% Get the NADH Image Button
% — Executes on button press in pushbutton1.
function read_NADH_intensity_Callback(~, ~, handles)
% hObject handle to pushbutton1 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global nadhTextImage;
global maskShowImage;
global nadhImageName;
global nadhFileName;
global nadhPathName;
% Get the location of the image
[nadhFileName, nadhPathName] = uigetfile(‘*.asc’,’Pick an Image’);
nadhTextImage = importdata(strcat(nadhPathName,nadhFileName));
nadhTextImage(nadhTextImage > 1000) = 0;
nadhTextImage(:,245:256)=0;
maskShowImage = nadhTextImage;
nadhImageName = {nadhFileName,’NADH’};
% display the NADH image in axes1
maxIntensityValue = max(nadhTextImage(:));
minIntensityValue = min(nadhTextImage(:));
set(handles.slider3,’Max’,maxIntensityValue);
set(handles.slider3,’Min’,minIntensityValue);
set(handles.text19,’String’,num2str(minIntensityValue));
set(handles.text20,’String’,num2str(maxIntensityValue));
set(handles.text23,’String’,nadhFileName);
axes(handles.axes1);
imagesc(nadhTextImage)
colormap(gray)
colorbar
axes(handles.axes4);
imagesc(maskShowImage)
colormap(gray)
colorbar
%% Get the Mask Image Button
% — Executes on button press in pushbutton4.
function pushbutton4_Callback(hObject, eventdata, handles)
% hObject handle to pushbutton4 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global nadhTextImage;
global maskImage;
global nadhThreshold;
% get the image mask
maskImage = nadhTextImage;
nadhThreshold = get(handles.edit1,’String’);
nadhThreshold = str2double( nadhThreshold );
maskImage(maskImage < nadhThreshold) = 0;
maskImage(maskImage~=0) = 1;
% display the mask Image axes4
axes(handles.axes4);
imagesc(maskImage)
colormap(gray)
colorbar

%% read FAD intensity image
% — Executes on button press in readFADIntensity.
function read_FAD_intensity_Callback(hObject, eventdata, handles)
% hObject handle to readFADIntensity (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global fadTextImage
% Get the location of the image
global fadImageName;
global fadFileName;
global fadPathName;
[fadFileName, fadPathName] = uigetfile(‘*.asc’,’Pick an Image’);
fadImageName = {fadFileName,’FAD’};
fadTextImage = importdata(strcat(fadPathName,fadFileName));
fadTextImage(fadTextImage > 1000) = 0;
fadTextImage(:,245:256)=0;
% display the NADH image in axes1
set(handles.text24,’String’,fadFileName);
axes(handles.axes2);
imagesc(fadTextImage)
colormap(gray)
colorbar
%% Read NADH T1 Image
% — Executes on button press in pushbutton3.
function pushbutton3_Callback(hObject, eventdata, handles)
% hObject handle to pushbutton3 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global nadhT1Image;
% Get the location of the image
[nadhT1FileName, nadhT1PathName] = uigetfile(‘*.asc’,’Pick an Image’);
nadhT1Image = importdata(strcat(nadhT1PathName,nadhT1FileName));
nadhT1Image(nadhT1Image > 8000) = 0;
nadhT1Image(:,245:256)=0;
% display the NADH image in axes7 and show the image name
axes(handles.axes7);
imagesc(nadhT1Image)
colormap(gray)
colorbar
set(handles.text7 , ‘String’, nadhT1FileName);
function edit1_Callback(hObject, eventdata, handles)
% hObject handle to edit1 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit1 as text
% str2double(get(hObject,’String’)) returns contents of edit1 as a double
% — Executes during object creation, after setting all properties.
function edit1_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit1 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
% — Executes during object deletion, before destroying properties.
function edit1_DeleteFcn(hObject, eventdata, handles)
% hObject handle to edit1 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
%% Calculate Redox Ratio Button
% — Executes on button press in pushbutton5.
function pushbutton5_Callback(hObject, eventdata, handles)
% hObject handle to pushbutton5 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global nadhTextImage;
global fadTextImage;
global redoxRatioImage;
redoxRatioImage = nadhTextImage./(nadhTextImage + fadTextImage);
axes(handles.axes6);
imagesc(redoxRatioImage)
colormap(gray)
colorbar
function edit2_Callback(hObject, eventdata, handles)
% hObject handle to edit2 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit2 as text
% str2double(get(hObject,’String’)) returns contents of edit2 as a double
% — Executes during object creation, after setting all properties.
function edit2_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit2 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
% — Executes on button press in pushbutton6.
function pushbutton6_Callback(~, ~, handles)
% hObject handle to pushbutton6 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global nadhTextImage;
global fadTextImage;
global redoxRatioImage;
global maskImage;
global redoxRatio;
nadhIntensityImage = nadhTextImage.* maskImage;
fadIntensityImage = fadTextImage.* maskImage;
axes(handles.axes1);
imagesc(nadhIntensityImage)
colormap(gray)
colorbar
axes(handles.axes2);
imagesc(fadIntensityImage)
colormap(gray)
colorbar
redoxRatioIntensityImage = redoxRatioImage.* maskImage;
axes(handles.axes6);
imagesc(redoxRatioIntensityImage)
colormap(gray)
colorbar
redoxRatio = mean(redoxRatioIntensityImage(redoxRatioIntensityImage~=0),’omitnan’);
set(handles.edit2 , ‘String’, num2str(redoxRatio));
%% Read NADH T2 Image and display
% — Executes on button press in pushbutton7.
function pushbutton7_Callback(hObject, eventdata, handles)
% hObject handle to pushbutton7 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global nadhT2Image;
% Get the location of the image
[nadhT2FileName, nadhT2PathName] = uigetfile(‘*.asc’,’Pick an Image’);
nadhT2Image = importdata(strcat(nadhT2PathName,nadhT2FileName));
nadhT2Image(nadhT2Image > 8000) = 0;
nadhT2Image(:,245:256)=0;
% display the NADH image in axes7 and show the image name
axes(handles.axes8);
imagesc(nadhT2Image)
colormap(gray)
colorbar
set(handles.text8 , ‘String’, nadhT2FileName);
%% Read NADH alpha Image and display
% — Executes on button press in pushbutton8.
function pushbutton8_Callback(hObject, eventdata, handles)
% hObject handle to pushbutton8 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global nadhAlphaImage;
% Get the location of the image
[nadhAlphaFileName, nadhAlphaPathName] = uigetfile(‘*.asc’,’Pick an Image’);
nadhAlphaImage = importdata(strcat(nadhAlphaPathName,nadhAlphaFileName));
nadhAlphaImage(nadhAlphaImage>100) = 0;
nadhAlphaImage(nadhAlphaImage<0) = 0;
nadhAlphaImage(:,245:256) = 0;
% display the NADH image in axes7 and show the image name
axes(handles.axes9);
imagesc(nadhAlphaImage)
colormap(gray)
colorbar
set(handles.text9 , ‘String’, nadhAlphaFileName);
%% Calculate NADH FLIM mean
% — Executes on button press in pushbutton9.
function pushbutton9_Callback(~, eventdata, handles)
% hObject handle to pushbutton9 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global nadhT1Image;
global nadhT2Image;
global nadhAlphaImage;
global maskImage;
global nadhT1Mean;
global nadhT2Mean;
global nadhAlphaMean;
global nadhFLIMMMean;
nadhFLIMMImage = (nadhT1Image.* nadhAlphaImage + nadhT2Image.*(100 – nadhAlphaImage))/100;
nadhFLIMMImageM = nadhFLIMMImage .* maskImage;
nadhAlphaImageM = nadhAlphaImage.* maskImage;
nadhT2ImageM = nadhT2Image .* maskImage;
nadhT1ImageM = nadhT1Image .* maskImage;
nadhAlphaMean = mean(nadhAlphaImageM(nadhAlphaImageM~=0),’omitnan’);
nadhT2Mean = mean(nadhT2ImageM(nadhT2ImageM~=0),’omitnan’);
nadhT1Mean = mean(nadhT1ImageM(nadhT1ImageM~=0),’omitnan’);
nadhFLIMMMean = mean(nadhFLIMMImageM(nadhFLIMMImageM~=0),’omitnan’);
set(handles.edit3 , ‘String’, num2str(nadhT1Mean));
set(handles.edit4 , ‘String’, num2str(nadhT2Mean));
set(handles.edit5 , ‘String’, num2str(nadhAlphaMean));
set(handles.edit6 , ‘String’, num2str(nadhFLIMMMean));
axes(handles.axes7);
imagesc(nadhT1ImageM)
colormap(gray)
colorbar
axes(handles.axes8);
imagesc(nadhT2ImageM)
colormap(gray)
colorbar
axes(handles.axes9);
imagesc(nadhAlphaImageM)
colormap(gray)
colorbar
function edit3_Callback(hObject, eventdata, handles)
% hObject handle to edit3 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit3 as text
% str2double(get(hObject,’String’)) returns contents of edit3 as a double
% — Executes during object creation, after setting all properties.
function edit3_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit3 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
function edit4_Callback(hObject, ~, handles)
% hObject handle to edit4 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit4 as text
% str2double(get(hObject,’String’)) returns contents of edit4 as a double
% — Executes during object creation, after setting all properties.
function edit4_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit4 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
function edit5_Callback(hObject, eventdata, handles)
% hObject handle to edit5 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit5 as text
% str2double(get(hObject,’String’)) returns contents of edit5 as a double
% — Executes during object creation, after setting all properties.
function edit5_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit5 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
function edit6_Callback(hObject, eventdata, handles)
% hObject handle to edit6 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit6 as text
% str2double(get(hObject,’String’)) returns contents of edit6 as a double
% — Executes during object creation, after setting all properties.
function edit6_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit6 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
%% Read FAD T1 image and display
% — Executes on button press in pushbutton10.
function pushbutton10_Callback(hObject, eventdata, handles)
% hObject handle to pushbutton10 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global fadT1Image;
% Get the location of the image
[fadT1FileName, fadT1PathName] = uigetfile(‘*.asc’,’Pick an Image’);
fadT1Image = importdata(strcat(fadT1PathName,fadT1FileName));
fadT1Image(fadT1Image > 8000) = 0;
fadT1Image(fadT1Image < 0) = 0;
fadT1Image(:,245:256)=0;
% display the NADH image in axes7 and show the image name
axes(handles.axes10);
imagesc(fadT1Image)
colormap(gray)
colorbar
set(handles.text10 , ‘String’, fadT1FileName);
% — Executes on button press in pushbutton11.
function pushbutton11_Callback(hObject, ~, handles)
% hObject handle to pushbutton11 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global fadT2Image;
% Get the location of the image
[fadT2FileName, fadT2PathName] = uigetfile(‘*.asc’,’Pick an Image’);
fadT2Image = importdata(strcat(fadT2PathName,fadT2FileName));
fadT2Image(fadT2Image>8000) = 0;
fadT2Image(fadT2Image < 0) = 0;
fadT2Image(:,245:256)=0;
% display the NADH image in axes7 and show the image name
axes(handles.axes11);
imagesc(fadT2Image)
colormap(gray)
colorbar
set(handles.text11 , ‘String’, fadT2FileName);
% — Executes on button press in pushbutton12.
function pushbutton12_Callback(~, ~, handles)
% hObject handle to pushbutton12 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global fadAlphaImage;
% Get the location of the image
[fadAplhaFileName, fadAlphaPathName] = uigetfile(‘*.asc’,’Pick an Image’);
fadAlphaImage = importdata(strcat(fadAlphaPathName,fadAplhaFileName));
fadAlphaImage(fadAlphaImage>100) = 0;
fadAlphaImage(fadAlphaImage<0) = 0;
fadAlphaImage(:,245:256)=0;
% display the NADH image in axes7 and show the image name
axes(handles.axes12);
imagesc(fadAlphaImage)
colormap(gray)
colorbar
set(handles.text12 , ‘String’, fadAplhaFileName);
% — Executes on button press in pushbutton13.
function pushbutton13_Callback(~, eventdata, handles)
% hObject handle to pushbutton13 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global fadT1Image;
global fadT2Image;
global fadAlphaImage;
global maskImage
global fadT1Mean;
global fadT2Mean;
global fadAlphaMean;
global fadFLIMMMean;
fadFLIMMImage = (fadT1Image.* fadAlphaImage + fadT2Image.*(100 – fadAlphaImage))/100;
fadFLIMMImageM = fadFLIMMImage .* maskImage;
fadAlphaImageM = fadAlphaImage.* maskImage;
fadT2ImageM = fadT2Image .* maskImage;
fadT1ImageM = fadT1Image .* maskImage;
fadAlphaMean = mean(fadAlphaImageM(fadAlphaImageM~=0),’omitnan’);
fadT2Mean = mean(fadT2ImageM(fadT2ImageM~=0),’omitnan’);
fadT1Mean = mean(fadT1ImageM(fadT1ImageM~=0),’omitnan’);
fadFLIMMMean = mean(fadFLIMMImageM(fadFLIMMImageM~=0),’omitnan’);
set(handles.edit7 , ‘String’, num2str(fadT1Mean));
set(handles.edit8 , ‘String’, num2str(fadT2Mean));
set(handles.edit9 , ‘String’, num2str(fadAlphaMean));
set(handles.edit10 , ‘String’, num2str(fadFLIMMMean));
axes(handles.axes10);
imagesc(fadT1ImageM)
colormap(gray)
colorbar
axes(handles.axes11);
imagesc(fadT2ImageM)
colormap(gray)
colorbar
axes(handles.axes12);
imagesc(fadAlphaImageM)
colormap(gray)
colorbar
function edit7_Callback(hObject, eventdata, handles)
% hObject handle to edit7 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit7 as text
% str2double(get(hObject,’String’)) returns contents of edit7 as a double
% — Executes during object creation, after setting all properties.
function edit7_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit7 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
function edit8_Callback(hObject, eventdata, handles)
% hObject handle to edit8 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit8 as text
% str2double(get(hObject,’String’)) returns contents of edit8 as a double
% — Executes during object creation, after setting all properties.
function edit8_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit8 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
function edit9_Callback(hObject, eventdata, handles)
% hObject handle to edit9 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit9 as text
% str2double(get(hObject,’String’)) returns contents of edit9 as a double
% — Executes during object creation, after setting all properties.
function edit9_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit9 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
function edit10_Callback(hObject, eventdata, handles)
% hObject handle to edit10 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit10 as text
% str2double(get(hObject,’String’)) returns contents of edit10 as a double
% — Executes during object creation, after setting all properties.
function edit10_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit10 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
function edit11_Callback(~, eventdata, handles)
% hObject handle to edit11 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit11 as text
% str2double(get(hObject,’String’)) returns contents of edit11 as a double
% — Executes during object creation, after setting all properties.
function edit11_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit11 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
function edit12_Callback(hObject, eventdata, handles)
% hObject handle to edit12 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit12 as text
% str2double(get(hObject,’String’)) returns contents of edit12 as a double
% — Executes during object creation, after setting all properties.
function edit12_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit12 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
function edit13_Callback(hObject, eventdata, handles)
% hObject handle to edit13 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit13 as text
% str2double(get(hObject,’String’)) returns contents of edit13 as a double
% — Executes during object creation, after setting all properties.
function edit13_CreateFcn(hObject, ~, handles)
% hObject handle to edit13 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
%% Select the file to save
% — Executes on button press in pushbutton15.
function pushbutton15_Callback(hObject, eventdata, handles)
% hObject handle to pushbutton15 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global saveExcelLocation;
[saveExcelName, saveExcelPath] = uigetfile(‘*.xlsx’,’Pick an excel file’);
saveExcelLocation = strcat(saveExcelPath,saveExcelName);
set(handles.edit14 , ‘String’, saveExcelLocation);
function edit14_Callback(hObject, eventdata, handles)
% hObject handle to edit14 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’String’) returns contents of edit14 as text
% str2double(get(hObject,’String’)) returns contents of edit14 as a double
% — Executes during object creation, after setting all properties.
function edit14_CreateFcn(hObject, eventdata, handles)
% hObject handle to edit14 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: edit controls usually have a white background on Windows.
% See ISPC and COMPUTER.
if ispc && isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,’white’);
end
%% Save the data to the excel file
% — Executes on button press in pushbutton16.
function pushbutton16_Callback(hObject, eventdata, handles)
% hObject handle to pushbutton16 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global nadhT1Mean;
global nadhT2Mean;
global nadhAlphaMean;
global nadhFLIMMMean;
global fadT1Mean;
global fadT2Mean;
global fadAlphaMean;
global fadFLIMMMean;
global redoxRatio;
global nadhThreshold;
global saveExcelLocation;
global nadhImageName ;
global fadImageName;
nadhFlimData = [nadhThreshold,redoxRatio,nadhT1Mean,nadhT2Mean,nadhAlphaMean,nadhFLIMMMean];
fadFlimData = [fadT1Mean,fadT2Mean,fadAlphaMean,fadFLIMMMean];
excelData = xlsread(saveExcelLocation,’Sheet1′,’E:E’); % read the column of interest
lastRowNumber = length(excelData)+ 2;
nadhWriteLoc = strcat(‘C’,num2str(lastRowNumber));
nadhNameWriteLoc = strcat(‘A’,num2str(lastRowNumber));
% save NADH data to the excel file
xlswrite(saveExcelLocation,nadhFlimData,’sheet1′,nadhWriteLoc);
xlswrite(saveExcelLocation,nadhImageName,’sheet1′,nadhNameWriteLoc);
% save FAD data to the excel file
lastRowNumber = lastRowNumber + 1;
fadWriteLoc = strcat(‘E’,num2str(lastRowNumber));
fadNameWriteLoc = strcat(‘A’,num2str(lastRowNumber));
xlswrite(saveExcelLocation,fadFlimData,’sheet1′,fadWriteLoc);
xlswrite(saveExcelLocation,fadImageName,’sheet1′,fadNameWriteLoc);
% — Executes on slider movement.
function slider3_Callback(hObject, eventdata, handles)
% hObject handle to slider3 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Hints: get(hObject,’Value’) returns position of slider
% get(hObject,’Min’) and get(hObject,’Max’) to determine range of slider
global nadhThreshold;
global maskShowImage;
global nadhTextImage
nadhThreshold = get(handles.slider3 ,’Value’);
% get the image mask
maskShowImage = nadhTextImage;
maskShowImage(maskShowImage < nadhThreshold) = 0;
set(handles.edit1 , ‘String’, num2str(nadhThreshold));
axes(handles.axes4);
imagesc(maskShowImage)
colormap(gray)
colorbar
% — Executes during object creation, after setting all properties.
function slider1_CreateFcn(hObject, eventdata, handles)
% hObject handle to slider3 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: slider controls usually have a light gray background.
if isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,[.9 .9 .9]);
end
function slider3_CreateFcn(hObject, eventdata, handles)
% hObject handle to slider3 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles empty – handles not created until after all CreateFcns called
% Hint: slider controls usually have a light gray background.
if isequal(get(hObject,’BackgroundColor’), get(0,’defaultUicontrolBackgroundColor’))
set(hObject,’BackgroundColor’,[.9 .9 .9]);
end
% — Executes on button press in pushbutton17.
function pushbutton17_Callback(hObject, eventdata, handles)
% hObject handle to pushbutton17 (see GCBO)
% eventdata reserved – to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global nadhFileName;
global nadhPathName;
global nadhT1Image;
% Get the location of the image
nadhT1FileName = strrep(nadhFileName,’photons’,’t1′);
nadhT1PathName = nadhPathName;
nadhT1Image = importdata(strcat(nadhT1PathName,nadhT1FileName));
nadhT1Image(nadhT1Image > 8000) = 0;
nadhT1Image(:,245:256)=0;
% display the NADH image in axes7 and show the image name
axes(handles.axes7);
imagesc(nadhT1Image)
colormap(gray)
colorbar
set(handles.text7 , ‘String’, nadhT1FileName);
global nadhT2Image;
% Get the location of the image
nadhT2FileName = strrep(nadhFileName,’photons’,’t2′);
nadhT2PathName = nadhPathName;
nadhT2Image = importdata(strcat(nadhT2PathName,nadhT2FileName));
nadhT2Image(nadhT2Image > 8000) = 0;
nadhT2Image(:,245:256)=0;
% display the NADH image in axes7 and show the image name
axes(handles.axes8);
imagesc(nadhT2Image)
colormap(gray)
colorbar
set(handles.text8 , ‘String’, nadhT2FileName);
global nadhAlphaImage;
% Get the location of the image
nadhAlphaFileName = strrep(nadhFileName,’photons’,’a1[%]’);
nadhAlphaPathName = nadhPathName;
nadhAlphaImage = importdata(strcat(nadhAlphaPathName,nadhAlphaFileName));
nadhAlphaImage(nadhAlphaImage>100) = 0;
nadhAlphaImage(nadhAlphaImage<0) = 0;
nadhAlphaImage(:,245:256) = 0;
% display the NADH image in axes7 and show the image name
axes(handles.axes9);
imagesc(nadhAlphaImage)
colormap(gray)
colorbar
set(handles.text9 , ‘String’, nadhAlphaFileName);
global fadFileName;
global fadPathName;
global fadT1Image;
% Get the location of the image
fadT1FileName = strrep(fadFileName,’photons’,’t1′);
fadT1PathName = fadPathName;
fadT1Image = importdata(strcat(fadT1PathName,fadT1FileName));
fadT1Image(fadT1Image > 8000) = 0;
fadT1Image(fadT1Image < 0) = 0;
fadT1Image(:,245:256)=0;
% display the NADH image in axes7 and show the image name
axes(handles.axes10);
imagesc(fadT1Image)
colormap(gray)
colorbar
set(handles.text10 , ‘String’, fadT1FileName);
global fadT2Image;
% Get the location of the image
fadT2FileName = strrep(fadFileName,’photons’,’t2′);
fadT2PathName = fadPathName;
fadT2Image = importdata(strcat(fadT2PathName,fadT2FileName));
fadT2Image(fadT2Image>8000) = 0;
fadT2Image(fadT2Image < 0) = 0;
fadT2Image(:,245:256)=0;
% display the NADH image in axes7 and show the image name
axes(handles.axes11);
imagesc(fadT2Image)
colormap(gray)
colorbar
set(handles.text11 , ‘String’, fadT2FileName)
global fadAlphaImage;
% Get the location of the image
fadAplhaFileName = strrep(fadFileName,’photons’,’a1[%]’);
fadAlphaPathName = fadPathName;
fadAlphaImage = importdata(strcat(fadAlphaPathName,fadAplhaFileName));
fadAlphaImage(fadAlphaImage>100) = 0;
fadAlphaImage(fadAlphaImage<0) = 0;
fadAlphaImage(:,245:256)=0;
% display the NADH image in axes7 and show the image name
axes(handles.axes12);
imagesc(fadAlphaImage)
colormap(gray)
colorbar
set(handles.text12 , ‘String’, fadAplhaFileName); hobject, eventdata, handles MATLAB Answers — New Questions

​